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Rabbit抗原名称
ROBO1分子别名
Roundabout homolog 1; Deleted in U twenty twenty; H-Robo-1; DUTT1免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
Q9Y6N7克隆号
S-2931-169抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HepG2, PANC-1, HeLa, BxPC-3, A549, U-2 OS, mouse E14 embryo, rat E14 embryo纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:500 Hu ICC 1:500 Hu
ROBO1 is a ~165 kDa single-pass transmembrane receptor of the immunoglobulin superfamily whose extracellular region contains five Ig-like domains and three fibronectin repeats that bind the secreted SLIT1/2 ligands, while its cytoplasmic tail carries four conserved CC motifs (CC0-CC3) that recruit adaptors such as srGAPs, Ena/VASP and DCC to transduce repulsive or attractive signals regulating axon guidance at the CNS midline, neuronal precursor migration, angiogenesis and even tumor metastasis by modulating Rho-family GTPases and actin cytoskeleton dynamics.
免疫印迹
WB result of ROBO1 Recombinant Rabbit mAb
Primary antibody: ROBO1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: LoVo whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: PANC-1 whole cell lysate 20 µg
Lane 5: HeLa whole cell lysate 20 µg
Lane 6: BxPC-3 whole cell lysate 20 µg
Lane 7: A549 whole cell lysate 20 µg
Lane 8: U-2 OS whole cell lysate 20 µg
Negative control: LoVo whole cell lysate
Low expression control: MCF7 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 181 kDa
Observed MW: 260 kDaWB result of ROBO1 Recombinant Rabbit mAb
Primary antibody: ROBO1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse E14 embryo lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 181 kDa
Observed MW: 260 kDaWB result of ROBO1 Recombinant Rabbit mAb
Primary antibody: ROBO1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat E14 embryo lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 181 kDa
Observed MW: 260 kDa
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-ROBO1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of ROBO1 in tumor tissue.
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