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Rabbit抗原名称
VE-Cadherin分子别名
Cadherin-5; 7B4 antigen; Vascular endothelial cadherin (VE-cadherin); CD144; CDH5免疫原
Synthetic Peptide细胞定位
Cell membrane, Cell junctionAccession
P33151克隆号
S-3312-5抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms, Rt阳性样本
bEnd.3, mouse lung, rat lung, rat heart纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20°C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt ICC 1:500 Ms
VE-Cadherin (vascular endothelial cadherin, CDH5) is a 140-kDa transmembrane glycoprotein that forms the core of endothelial adherens junctions, where its calcium-dependent homophilic interactions between extracellular EC1-EC5 repeats create a semi-permeable barrier between blood and tissues; its cytoplasmic tail recruits β-catenin, plakoglobin and p120-catenin, linking the junction to the actin cytoskeleton and enabling transmission of shear-stress signals that regulate angiogenesis, vascular remodeling and leukocyte extravasation, while phosphorylation of Y685 and Y658 by Src, VEGFR2 or Tie-2 and subsequent internalization of the complex transiently increases permeability during inflammation, tumor metastasis and atherosclerosis, making VE-cadherin a key therapeutic target in vascular pathologies.
免疫印迹
WB result of VE-Cadherin Recombinant Rabbit mAb
Primary antibody: VE-Cadherin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: bEnd.3 whole cell lysate 20 µg
Negative control: NIH/3T3 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 88 kDa
Observed MW: 88, 100-140 kDaWB result of VE-Cadherin Recombinant Rabbit mAb
Primary antibody: VE-Cadherin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse lung lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 88 kDa
Observed MW: 100-120 kDaWB result of VE-Cadherin Recombinant Rabbit mAb
Primary antibody: VE-Cadherin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat lung lysate 20 µg
Lane 2: rat heart lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 88 kDa
Observed MW: 100-140 kDa
免疫细胞化学
ICC shows positive staining in bEnd.3 cells (top panel) and negative staining in NIH/3T3 cells (below panel). Anti-VE-Cadherin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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