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Rabbit抗原名称
MCL1分子别名
Induced myeloid leukemia cell differentiation protein Mcl-1 homolog; Bcl-2-related protein EAT/mcl1; Mcl1免疫原
Synthetic Peptide细胞定位
Nucleus, Mitochondrion, CytoplasmAccession
P97287克隆号
S-2640-208抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms, Rt阳性样本
L-929, A20, C2C12, rat kidney, rat stomach纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt
MCL1 (Myeloid cell leukemia 1) is an anti-apoptotic member of the BCL-2 protein family that plays a crucial role in regulating cell survival and death by binding to and neutralizing pro-apoptotic proteins such as BAK and BIM, thereby preventing mitochondrial outer membrane permeabilization and subsequent activation of the intrinsic apoptotic pathway.
免疫印迹
WB result of MCL1 Recombinant Rabbit mAb
Primary antibody: MCL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: L-929 whole cell lysate 20 µg
Lane 2: A20 whole cell lysate 20 µg
Lane 3: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 35 kDa
Observed MW: 37 kDaWB result of MCL1 Recombinant Rabbit mAb
Primary antibody: MCL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat stomach lysate 20 µg
Lane 2: rat kidney lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 35 kDa
Observed MW: 37 kDa
This blot was developed with high sensitivity substrate
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