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Rabbit抗原名称
MBD3分子别名
Methyl-CpG-binding domain protein 3; Methyl-CpG-binding protein MBD3细胞定位
NucleusAccession
O95983克隆号
S-4367抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
K562, NTERA-2, HeLa, 293T, F9, NIH/3T3, C6, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk ICC 1:500 Hu
MBD3 is a 32-kDa core subunit of the nuclear Mi-2/NuRD chromatin-remodeling complex that is essential for embryonic development in mice; it binds 5-methyl-cytosine DNA indirectly through a degenerate methyl-CpG-binding domain, couples ATP-dependent nucleosome sliding with histone deacetylase activity, and thereby orchestrates transcriptional repression, pluripotency exit, lineage specification, and heterochromatin formation across vertebrate genomes.
免疫印迹
WB result of MBD3 Recombinant Rabbit mAb
Primary antibody: MBD3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: NTERA-2 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: 293T whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 32, 36 kDaWB result of MBD3 Recombinant Rabbit mAb
Primary antibody: MBD3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: F9 whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 32, 36 kDaWB result of MBD3 Recombinant Rabbit mAb
Primary antibody: MBD3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 32, 36 kDaWB result of MBD3 Recombinant Rabbit mAb
Primary antibody: MBD3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 32, 36 kDa
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- MBD3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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