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宿主来源
Rabbit抗原名称
Histone H3 (tri methyl K27)分子别名
H3K27me3细胞定位
NucleusAccession
P68431克隆号
S-4331抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
甲基化反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HeLa, Jurkat, NIH/3T3, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ChIP
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu, Ms, Mk IHC-P 1:1000 Hu, Ms, Rt ICC 1:1000 Hu, Ms ChIP 1:20-1:50 Hu
Histone H3 (tri methyl K27) (H3K27me3) is a crucial epigenetic modification catalyzed by the Polycomb Repressive Complex 2 (PRC2). It functions primarily in the transcriptional repression of target genes and plays a key role in biological processes such as embryonic development, cell fate determination, and genomic imprinting. It is particularly vital for maintaining stem cell pluripotency and establishing cell type-specific gene expression patterns. Aberrant distribution of H3K27me3 is associated with various diseases, including developmental disorders and cancers (such as certain sarcomas and lymphomas), where its excessive silencing can lead to the inactivation of tumor suppressor genes. Common techniques for detecting H3K27me3 include ChIP-seq and immunofluorescence. Furthermore, it is a significant target for current epigenetic drug development, such as EZH2 inhibitors.
免疫印迹
WB result of Histone H3 (tri methyl K27) Recombinant Rabbit mAb
Primary antibody: Histone H3 (tri methyl K27) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
This blot was developed with high sensitivity substrateWB result of Histone H3 (tri methyl K27) Recombinant Rabbit mAb
Primary antibody: Histone H3 (tri methyl K27) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
This blot was developed with high sensitivity substrateWB result of Histone H3 (tri methyl K27) Recombinant Rabbit mAb
Primary antibody: Histone H3 (tri methyl K27) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-Histone H3 (tri methyl K27) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Histone H3 (tri methyl K27) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-Histone H3 (tri methyl K27) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-Histone H3 (tri methyl K27) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-Histone H3 (tri methyl K27) antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti-Histone H3 (tri methyl K27) antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross -linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Histone H3 (tri methyl K27) Recombinant Rabbit mAb (S-4331) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
Post-immunoprecipitation, both samples
were washed, eluted, and cross-links reversed. Purified DNA was analyzed by qPCR.
qPCR
showed the enrichment of RPL30, GAPDH, MYOD1,
AFM, SAT-α and SAT-2 in Histone H3 (tri methyl K27)
Recombinant Rabbit mAb (S-4331)-immunoprecipitated
sample.
组织表达图谱
Expression of Histone H3 (tri methyl K27) in tumor tissue.
Expression of Histone H3 (tri methyl K27) in human tissue.
Expression of Histone H3 (tri methyl K27) in mouse & rat tissue.
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