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宿主来源
Rabbit抗原名称
Galactosylceramidase分子别名
Galactocerebrosidase; GALCERase; Galactocerebroside beta-galactosidase; Galactosylceramide beta-galactosidase; Galc免疫原
Recombinant Protein细胞定位
LysosomeAccession
P54818克隆号
S-3402-17抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
A549, SH-SY5Y, A375, HepG2, mouse brain, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Mk IHC-P 1:1000 Hu, Ms, Rt
Galactosylceramidase, also known as galactocerebrosidase (GALC), is a lysosomal enzyme encoded by the GALC gene on chromosome 14q31.3 that catalyzes the hydrolytic cleavage of galactose from galactosylceramide and other galactolipids, playing a crucial role in myelin maintenance and turnover; deficiency in this enzyme leads to the accumulation of toxic metabolites, particularly psychosine, resulting in Krabbe disease (globoid cell leukodystrophy), a severe neurodegenerative disorder characterized by demyelination, multinucleated globoid cells in the white matter, and progressive loss of motor and cognitive functions, with early-onset forms typically manifesting within the first six months of life and proving fatal within a few years.
免疫印迹
WB result of Galactosylceramidase Recombinant Rabbit mAb
Primary antibody: Galactosylceramidase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Lane 2: SH-SY5Y whole cell lysate 20 µg
Lane 3: A375 whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 77 kDa
Observed MW: 30, 50, 70, 170 kDaWB result of Galactosylceramidase Recombinant Rabbit mAb
Primary antibody: Galactosylceramidase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 77 kDa
Observed MW: 30, 50, 65 kDaWB result of Galactosylceramidase Recombinant Rabbit mAb
Primary antibody: Galactosylceramidase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 77 kDa
Observed MW: 30, 50 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Galactosylceramidase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Galactosylceramidase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Galactosylceramidase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Galactosylceramidase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Galactosylceramidase in tumor tissue.
Expression of Galactosylceramidase in human tissue.
Expression of Galactosylceramidase in mouse & rat tissue.
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