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Rabbit抗原名称
GABRA1分子别名
Gamma-aminobutyric acid receptor subunit alpha-1; GABA(A) receptor subunit alpha-1 (GABAAR subunit alpha-1); Gabra-1; Gabra1免疫原
Synthetic Peptide细胞定位
Cell membrane, SynapseAccession
P62813克隆号
S-3254-10抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
mouse brain, rat brain预测反应种属
(反应种属缩写表)CyMk, Bv, Or, Ck纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:1000 Hu, Ms, Rt IF 1:500 Ms, Rt
GABRA1 (gamma-aminobutyric acid type A receptor subunit alpha1) is the most abundant α-subunit of the pentameric GABA_A receptor, the brain’s principal fast inhibitory ion channel, and its TM2 helix lines the chloride-selective pore; mutations like A322D or E177A reduce surface expression or gating, causing severe epileptic encephalopathy, while single-nucleotide polymorphisms modulate sensitivity to benzodiazepines, ethanol and general anesthetics that bind at the α1/γ2 interface, making GABRA1 a pharmacological cornerstone for sedation, sleep and anesthesia as well as a high-priority target for precision therapy of genetic epilepsy.
免疫印迹
WB result of GABRA1 Recombinant Rabbit mAb
Primary antibody: GABRA1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse lung lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Negative control: mouse lung lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 52 kDaWB result of GABRA1 Recombinant Rabbit mAb
Primary antibody: GABRA1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat lung lysate 20 µg
Lane 2: rat brain lysate 20 µg
Negative control: rat lung lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 52 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-GABRA1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-GABRA1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human ovarian cancer. Anti-GABRA1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-GABRA1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-GABRA1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-GABRA1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat cerebral cortex. Anti-GABRA1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of GABRA1 in tumor tissue.
Expression of GABRA1 in human tissue.
Expression of GABRA1 in mouse & rat tissue.
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