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CD206 Recombinant Rabbit mAb (S-3477-27)

Macrophage mannose receptor 1,MMR,Mrc1

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6667
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    CD206

  • 分子别名

    Macrophage mannose receptor 1; MMR; Mrc1

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Endosome, Cell membrane

  • Accession

    Q61830

  • 克隆号

    S-3477-27

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    PMA, IL-4 and Brefeldin A treated THP-1, mouse liver, mouse lung, mouse spleen, rat liver, rat lung, rat spleen

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    WB

    IF ?

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms, Rt
    IHC-P 1:2000-1:5000 Hu, Ms, Rt
    IF 1:2000 Hu, Ms
背景介绍

  • CD206, also known as the mannose receptor (MR) or mannose receptor C type 1 (MRC1), is a transmembrane glycoprotein that belongs to the C-type lectin family. It is predominantly expressed by tissue macrophages, dendritic cells, and certain lymphatic or liver endothelial cells. CD206 recognizes and mediates endocytosis of pathogens by binding to glycoproteins terminated with mannose. This receptor serves as a homeostatic receptor by binding and scavenging unwanted high mannose N-linked glycoproteins as well as pituitary hormones from the circulation. In addition, CD206 is a marker for the M2 phenotype of macrophages, which are involved in immunosuppression, tissue remodeling, and Th2 effector responses. Its expression is regulated by various cytokines and is considered a marker of an M2-like macrophage phenotype.

  • 免疫印迹

    • WB result of CD206 Recombinant Rabbit mAb
      Primary antibody: CD206 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated THP-1 whole cell lysate 20 µg
      Lane 2: THP-1 treated with 100 ng/ml PMA for 24 hours, then treated with 20 ng/ml IL-4 and 20 ng/ml IL-13 for 72 hours, then treated with 300 ng/ml Brefeldin A for last 4 hours whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 165 kDa
      Observed MW: 220 kDa
      This blot was developed with high sensitivity substrate

    • WB result of CD206 Recombinant Rabbit mAb
      Primary antibody: CD206 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: mouse brain lysate 20 µg
      Lane 2: mouse liver lysate 20 µg
      Lane 3: mouse lung lysate 20 µg
      Lane 4: mouse spleen lysate 20 µg
      Low expression control: mouse brain lysate
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 165 kDa
      Observed MW: 220 kDa

    • WB result of CD206 Recombinant Rabbit mAb
      Primary antibody: CD206 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: rat brain lysate 20 µg
      Lane 2: rat liver lysate 20 µg
      Lane 3: rat lung lysate 20 µg
      Lane 4: rat spleen lysate 20 µg
      Low expression control: rat brain lysate
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 165 kDa
      Observed MW: 220 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human spleen. Anti-CD206 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human placenta. Anti-CD206 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human liver. Anti-CD206 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-CD206 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse colon. Anti-CD206 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse liver. Anti-CD206 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat lung. Anti-CD206 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat liver. Anti-CD206 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫荧光

    • IF shows positive staining in paraffin-embedded human spleen. Anti-CD206 antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • Negative control: IF shows negative staining in paraffin-embedded human brain. Anti-CD206 antibody was used at 1/2000 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • IF shows positive staining in paraffin-embedded mouse spleen. Anti-CD206 antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • Negative control: IF shows negative staining in paraffin-embedded mouse brain. Anti-CD206 antibody was used at 1/2000 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

  • 组织表达图谱

    • Expression of CD206 in tumor tissue.

    • Expression of CD206 in human tissue.

    • Expression of CD206 in mouse & rat tissue.

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