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Rabbit抗原名称
SLC31A1分子别名
High affinity copper uptake protein 1; Copper transporter 1 (hCTR1); Solute carrier family 31 member 1; COPT1; CTR1; SLC31A1免疫原
Synthetic Peptide细胞定位
Cell membrane, EndosomeAccession
O15431抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
Caco-2, Ramos, HepG2, HT-29, mouse brain, rat brain预测反应种属
(反应种属缩写表)Or, Pg纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB Hu, Ms, Rt
SLC31A1 (also known as CTR1) is a high-affinity, plasma-membrane copper importer that drives cuprous ion (Cu⁺) uptake by forming a homotrimeric channel, binds blood Cu²⁺-albumin at its N-terminal domain to initiate reduction and vectorial transport, hands Cu⁺ to the chaperone ATOX1 via its C-terminal HCH motif, is transcriptionally repressed by Cu-loaded Sp1 yet induced by hypoxia through HIF-2α/EPAS1, undergoes rapid endocytosis and degradation when intracellular copper rises, doubles as a saturable gate for cisplatin, silver and cadmium uptake, triggers cuproptosis when over-expressed, and in endothelial cells acts as a redox sensor that transmits VEGF-generated ROS through Cys-189 sulfenylation to form a disulfide-linked SLC31A1–KDR complex whose co-internalization sustains VEGFR2 signaling and angiogenesis independent of copper transport.
免疫印迹
WB result of SLC31A1 Rabbit pAb
Primary antibody: SLC31A1 Rabbit pAb at 1/1000 dilution
Primary antibody incubation conditions: overnight at 4°C
Lane 1: unboiled Caco-2 whole cell lysate 20 µg
Lane 2: unboiled Ramos whole cell lysate 20 µg
Lane 3: unboiled HepG2 whole cell lysate 20 µg
Lane 4: unboiled HT-29 whole cell lysate 20 µg
Lane 5: unboiled Caco-2 (deglycosylated with PNGase F) whole cell lysate 20 µg
Lane 6: unboiled Ramos (deglycosylated with PNGase F) whole cell lysate 20 µg
Lane 7: unboiled HepG2 (deglycosylated with PNGase F) whole cell lysate 20 µg
Lane 8: unboiled HT-29 (deglycosylated with PNGase F) whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 26, 30, 52, 60 kDaWB result of SLC31A1 Rabbit pAb
Primary antibody: SLC31A1 Rabbit pAb at 1/1000 dilution
Primary antibody incubation conditions: overnight at 4°C
Lane 1: unboiled mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 26, 52 kDaWB result of SLC31A1 Rabbit pAb
Primary antibody: SLC31A1 Rabbit pAb at 1/1000 dilution
Primary antibody incubation conditions: overnight at 4°C
Lane 1: unboiled rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 26, 52 kDa
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