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宿主来源
Rabbit抗原名称
Phospho-TrkA (Tyr490)/TrkB (Tyr516)分子别名
High affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type; Tyrosine kinase receptor; TRK细胞定位
Cell membrane, EndosomeAccession
P04629, Q16620克隆号
S-3812抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu, Rt阳性样本
β-NGF treated PC-12纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Rt
Phospho-TrkA (Tyr490) and phospho-TrkB (Tyr516) are the neurotrophin-activated, autophosphorylated forms of the receptor tyrosine kinases TrkA and TrkB; once these juxtamembrane tyrosines are phosphorylated they serve as docking platforms for adaptor proteins—TrkA-pTyr490 recruits FRS2 and Shc to trigger Ras/MAPK and PI3K/Akt cascades that mediate neuronal survival and differentiation, whereas TrkB-pTyr516 binds Shc to activate the same pathways plus PLCγ-driven PKC and Ca²⁺ signals that regulate synaptic plasticity—so antibodies detecting these phospho-sites are widely used as biomarkers for NGF- or BDNF-dependent Trk signaling in health and disease.
免疫印迹
WB result of Phospho-TrkA (Tyr490)/TrkB (Tyr516) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-TrkA (Tyr490)/TrkB (Tyr516) Recombinant Rabbit mAb at 1/1000 dilution
Primary antibody incubation conditions: overnight at 4°C
Lane 1: untreated PC-12 whole cell lysate 20 µg
Lane 2: PC-12 starved for 4 hours, then treated with 100 ng/ml β-NGF for 5 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 92 kDa
Observed MW: 150 kDa
This blot was developed with high sensitivity substrate
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