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宿主来源
Rabbit抗原名称
MLKL分子别名
Mixed lineage kinase domain-like protein; hMLKL; MLKL细胞定位
Cell membrane, Cytoplasm, NucleusAccession
Q8NB16克隆号
S-4248抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu阳性样本
Z-VAD, hTNF-α and SM-164 treated HT-29纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu
Mixed lineage kinase domain-like protein (MLKL) is a pseudokinase that belongs to the protein kinase superfamily and contains a protein kinase-like domain but lacks catalytic activity. It plays a crucial role in necroptosis, a form of regulated cell death. When activated by receptor-interacting protein kinase 3 (RIPK3), MLKL oligomerizes and translocates to the plasma membrane, causing membrane permeabilization and ultimately leading to cell death. Besides its canonical role in necroptosis, MLKL has been found to be associated with various cellular organelles, such as the mitochondrion, lysosome, endosome, endoplasmic reticulum, and nucleus, where it may regulate diverse pathophysiological events. Additionally, MLKL has necroptosis-independent functions in processes like receptor internalization, ligand-receptor degradation, endosomal trafficking, autophagy, and nuclear functions. Its involvement in multiple diseases and potential as a therapeutic target are also being actively investigated.
免疫印迹
WB result of Phospho-MLKL (Ser358) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-MLKL (Ser358) Recombinant Rabbit mAb at 1/1000 dilution
Primary antibody incubation conditions: overnight at 4°C
Lane 1: untreated HT-29 whole cell lysate 20 µg
Lane 2: HT-29 treated with 20 μM Z-VAD for 30 minutes, then add 20 ng/ml hTNF-α and 100 nM SM-164 for 7 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 54 kDa
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