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宿主来源
Rabbit抗原名称
ALP分子别名
PDZ and LIM domain protein 3; Actinin-associated LIM protein; Alpha-actinin-2-associated LIM protein; PDLIM3免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
Q53GG5克隆号
S-2786-41抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
U-2 OS, mouse skeletal muscle, rat skeletal muscle, rat heart预测反应种属
(反应种属缩写表)Pg, Bv纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu, Ms, Rt
Alkaline phosphatase (ALP) is a ubiquitous membrane-bound metalloenzyme that catalyzes the non-specific hydrolysis of phosphate monoesters at alkaline pH, existing as four distinct human isozymes—tissue-non-specific (liver/bone/kidney), intestinal, placental and germ-cell—whose differential glycosylation and post-translational modifications generate characteristic electrophoretic patterns exploited clinically to pinpoint the source of elevated serum activity that serves as a sensitive but non-specific biomarker for hepatobiliary obstruction, bone formation by osteoblasts, pregnancy, malignancy, or metabolic disorders, while intracellularly the enzyme participates in lipid transport, dephosphorylation of receptors, and modulation of inflammatory signaling through its ectophosphatase activity on extracellular ATP.
免疫印迹
WB result of ALP Recombinant Rabbit mAb
Primary antibody: ALP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: U-2 OS whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 37 kDa
This blot was developed with high sensitivity substrateWB result of ALP Recombinant Rabbit mAb
Primary antibody: ALP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse kidney lysate 20 µg
Lane 2: mouse skeletal muscle lysate 20 µg
Negative control: mouse kidney lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 37 kDa
This blot was developed with high sensitivity substrateWB result of ALP Recombinant Rabbit mAb
Primary antibody: ALP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat kidney lysate 20 µg
Lane 2: rat skeletal muscle lysate 20 µg
Lane 3: rat heart lysate 20 µg
Negative control: rat kidney lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 37 kDa
This blot was developed with high sensitivity substrate
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