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Rabbit抗原名称
α/β-Tubulin免疫原
Synthetic Peptide细胞定位
Cytoplasm, CytoskeletonAccession
P07437, P68363抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HeLa, A431, A549, NIH/3T3, mouse brain, C6, rat brain, COS-7纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:5000 Hu, Ms, Rt, Mk IHC-P 1:200 Hu, Ms, Rt ICC 1:500 Hu
The α/β-Tubulin heterodimer is the fundamental building block of microtubules. These proteins polymerize in a GTP-dependent manner to form the polarized protofilaments of the microtubule lattice. α-Tubulin typically binds GTP irreversibly, providing structural stability, while the hydrolysis of GTP to GDP on β-Tubulin is the key driver of microtubule dynamic instability—the stochastic switch between phases of growth and rapid disassembly. Both subunits share a highly conserved GTPase domain and interact longitudinally to extend the hollow cylindrical structure. They are essential for critical cellular processes including cell division, ciliary motility, and intracellular transport. The functionality of microtubules is precisely regulated by tubulin expression levels and post-translational modifications, such as acetylation and tyrosination. Consequently, α/β-tubulin is a major target for cancer chemotherapy (e.g., microtubule-targeting agents) and a focal point in neurodegenerative disease research.
免疫印迹
WB result of α/β-Tubulin Rabbit pAb
Primary antibody: α/β-Tubulin Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 52 kDaWB result of α/β-Tubulin Rabbit pAb
Primary antibody: α/β-Tubulin Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 52 kDaWB result of α/β-Tubulin Rabbit pAb
Primary antibody: α/β-Tubulin Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 52 kDaWB result of α/β-Tubulin Rabbit pAb
Primary antibody: α/β-Tubulin Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 52 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti- α/β-Tubulin antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti- α/β-Tubulin antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti- α/β-Tubulin antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti- α/β-Tubulin antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- α/β-Tubulin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
组织表达图谱
Expression ofα/β-Tubulin in tumor tissue.
Expression ofα/β-Tubulin in human tissue.
Expression ofα/β-Tubulin in mouse & rat tissue.
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