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Rabbit抗原名称
PDE4分子别名
3',5'-cyclic-AMP phosphodiesterase 4A; DPDE2; PDE46; cAMP-specific phosphodiesterase 4A; PDE4A免疫原
Synthetic Peptide细胞定位
Cytoplasm, Cell membraneAccession
P27815克隆号
S-2598-147抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, SH-SY5Y, mouse brain, mouse cerebellum, rat brain, rat cerebellum纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:100 Hu, Ms, Rt
PDE4 (phosphodiesterase-4) is a cAMP-specific phosphodiesterase that hydrolyzes the second messenger cyclic adenosine monophosphate (cAMP) to 5'-AMP, thereby terminating cAMP signaling cascades involved in inflammation, memory, cardiac contractility and immune cell regulation; the enzyme family comprises four subtypes (PDE4A-D) that differ in N-terminal targeting domains and regulatory Upstream Conserved Regions (UCR1/2) controlling dimerization and PKA-mediated phosphorylation, and selective inhibition of PDE4—for example with roflumilast or apremilast—elevates intracellular cAMP, suppressing pro-inflammatory cytokines (TNF-α, IL-23) and increasing anti-inflammatory IL-10, making PDE4 inhibitors clinically useful in psoriasis, psoriatic arthritis, Behçet’s disease and severe COPD.
免疫印迹
WB result of PDE4 Recombinant Rabbit mAb
Primary antibody: PDE4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: DU 145 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: SH-SY5Y whole cell lysate 20 µg
Negative control: K562 whole cell lysate; DU 145 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 98 kDa
Observed MW: 90 kDa
This blot was developed with high sensitivity substrateWB result of PDE4 Recombinant Rabbit mAb
Primary antibody: PDE4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse cerebellum lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 98 kDa
Observed MW: 80-110 kDaWB result of PDE4 Recombinant Rabbit mAb
Primary antibody: PDE4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat cerebellum lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 98 kDa
Observed MW: 80-100 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-PDE4 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-PDE4 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-PDE4 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of PDE4 in tumor tissue.
Expression of PDE4 in human tissue.
Expression of PDE4 in mouse & rat tissue.
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