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宿主来源
Rabbit抗原名称
GDF3分子别名
Growth/differentiation factor 3; GDF-3免疫原
Synthetic Peptide细胞定位
Cytoplasm, SecretedAccession
Q9NR23克隆号
S-3049-3抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
稀释度
应用 稀释度 推荐种属 IHC-P 1:100-1:250 Hu, Ms
GDF3 (growth differentiation factor 3) is a secreted, cysteine-knot-containing ligand of the TGF-β superfamily that acts as a context-dependent morphogen during early embryogenesis, axial patterning, and cartilage formation by binding specific type I/II receptor serine kinases to activate SMAD2/3 or SMAD1/5/8 signaling cascades, thereby regulating target genes that control cell fate, proliferation, and extracellular-matrix production; pathogenic missense variants (e.g., R84C, R84L, R266C) disrupt its biosynthesis, secretion, or receptor engagement and are strongly linked to congenital skeletal malformations such as Klippel-Feil syndrome and scoliosis, while in adipose tissue its expression is acutely up-regulated by cold exposure, suggesting a newly recognized role in adaptive thermogenesis.
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-GDF3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-GDF3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-GDF3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-GDF3 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse liver. Anti-GDF3 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of GDF3 in tumor tissue.
Expression of GDF3 in human tissue.
Expression of GDF3 in mouse & rat tissue.
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