EHMT2/G9A Recombinant Rabbit mAb (S-4200)
Histone-lysine N-methyltransferase EHMT2,Euchromatic histone-lysine N-methyltransferase 2,HLA-B-associated transcript 8,Histone H3-K9 methyltransferase 3 (H3-K9-HMTase 3),Lysine N-methyltransferase 1C,Protein G9a,BAT8,C6orf30,KMT1C,NG36
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宿主来源
Rabbit抗原名称
EHMT2/G9A分子别名
Histone-lysine N-methyltransferase EHMT2; Euchromatic histone-lysine N-methyltransferase 2; HLA-B-associated transcript 8; Histone H3-K9 methyltransferase 3 (H3-K9-HMTase 3); Lysine N-methyltransferase 1C; Protein G9a; BAT8; C6orf30; KMT1C; NG36细胞定位
NucleusAccession
Q96KQ7克隆号
S-4200抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
MCF7, HL-60, HEK-293, HT-1080, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ChIP
IHC-P ?
ICC ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Mk IHC-P 1:1000 Hu, Ms, Rt ICC 1:500 Hu IF 1:500 Hu ChIP 1:20-1:50 Hu
EHMT2 (also called G9a) is a nuclear lysine methyltransferase that chiefly di-methylates histone H3 at lysine 9 (H3K9me2) and lysine 27 (H3K27me1/2) to establish facultative heterochromatin and transcriptionally silence target genes, and it can act either as a repressor or, through auto-methylation-dependent recruitment of cofactors such as HP1γ and p300, as a co-activator of transcription; the enzyme exists as a homodimer or heterodimer with the related GLP/EHMT1, contains an N-terminal ankyrin-repeat domain for chromatin docking and a catalytic SET domain for methyl transfer, and its dysregulation—via overexpression in diverse solid and hematologic cancers or down-regulation in addiction and neuropathic pain—alters the expression of hundreds of genes by both histone and non-histone methylation, thereby controlling embryonic development, stem-cell lineage commitment, immune-cell differentiation, synaptic remodeling, and tumorigenicity .
免疫印迹
WB result of EHMT2/G9A Recombinant Rabbit mAb
Primary antibody: EHMT2/G9A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: HL-60 whole cell lysate 20 µg
Lane 3: HEK-293 whole cell lysate 20 µg
Lane 4: HT-1080 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 132 kDa
Observed MW: 130-180 kDaWB result of EHMT2/G9A Recombinant Rabbit mAb
Primary antibody: EHMT2/G9A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 132 kDa
Observed MW: 180 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-EHMT2/G9A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-EHMT2/G9A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-EHMT2/G9A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-EHMT2/G9A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-EHMT2/G9A antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
免疫荧光
IF shows positive staining in paraffin-embedded human ovarian cancer. Anti- EHMT2/G9A antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 647) (S0B4005) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human cervical cancer. Anti- EHMT2/G9A antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 647) (S0B4005) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
ChIP
Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used EHMT2/G9A Recombinant Rabbit mAb (S-4200) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
Post-immunoprecipitation, both samples were washed, eluted, and cross-links reversed. Purified DNA was analyzed by qPCR.
qPCR showed the enrichment of VRK3, ZNF19 and SAT-α in
EHMT2/G9A Recombinant Rabbit mAb (S-4200) -immunoprecipitated
sample.
组织表达图谱
Expression of EHMT2/G9A in tumor tissue.
Expression of EHMT2/G9A in human tissue.
Expression of EHMT2/G9A in mouse & rat tissue.
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