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宿主来源
Rabbit抗原名称
SMAD3分子别名
Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3 (SMAD 3; Smad3; hSMAD3); MADH3免疫原
Recombinant Protein细胞定位
Cytoplasm, NucleusAccession
P84022克隆号
S-3147-36抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HT-1080, HeLa, Jurkat, K562, A431, RAW264.7, C6, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk IHC-P 1:1000 Hu, Ms ICC 1:500 Hu
SMAD3 protein is a key intracellular signaling transducer in the Transforming Growth Factor-β (TGF-β) signaling pathway. As one of the receptor-regulated SMADs (R-SMADs), SMAD3 is phosphorylated and activated upon binding of TGF-β superfamily ligands (such as TGF-β and activin) to their cell membrane receptors. It then forms a complex with SMAD4 and translocates into the nucleus, where it functions as a transcription factor to directly regulate the expression of target genes. It is extensively involved in physiological processes such as cell proliferation, differentiation, apoptosis, extracellular matrix formation, and immune regulation. SMAD3 plays a dual role in various pathological conditions, including fibrosis, cancer, and inflammatory diseases (it can suppress early tumor growth but also promote late-stage tumor progression and metastasis). Its activity is finely regulated by post-translational modifications such as ubiquitination and acetylation, as well as by inhibitory SMADs (e.g., SMAD7), making it an important potential therapeutic target for diseases.
免疫印迹
WB result of SMAD3 Recombinant Rabbit mAb
Primary antibody: SMAD3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HT-1080 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Lane 5: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 55 kDaWB result of SMAD3 Recombinant Rabbit mAb
Primary antibody: SMAD3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 55 kDaWB result of SMAD3 Recombinant Rabbit mAb
Primary antibody: SMAD3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 55 kDaWB result of SMAD3 Recombinant Rabbit mAb
Primary antibody: SMAD3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 55 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-SMAD3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-SMAD3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-SMAD3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-SMAD3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- Smad3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of SMAD3 in tumor tissue.
Expression of SMAD3 in human tissue.
Expression of SMAD3 in mouse & rat tissue.
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