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Rabbit抗原名称
PKC beta分子别名
Protein kinase C beta type; PKC-B; PKC-beta; PKCB; PRKCB1; PRKCB免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
P05771克隆号
S-3158-28抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
K562, THP-1, mouse brain, rat brain纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt ICC 1:500 Hu, Ms, Rt
Protein kinase C beta (PKCβ) is a serine/threonine kinase of the conventional PKC subfamily that is activated by diacylglycerol and calcium; it phosphorylates diverse substrates to regulate glucose transport, vascular permeability, immune cell signaling and cell survival, and its sustained overactivity drives diabetic microvascular complications, atherosclerosis and certain cancers, making selective PKCβ inhibitors attractive therapeutic candidates.
免疫印迹
WB result of PKC beta Recombinant Rabbit mAb
Primary antibody: PKC beta Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HCT 116 whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: THP-1 whole cell lysate 20 µg
Negative control: HCT 116 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 77 kDa
Observed MW: 77 kDaWB result of PKC beta Recombinant Rabbit mAb
Primary antibody: PKC beta Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 77 kDa
Observed MW: 77 kDaWB result of PKC beta Recombinant Rabbit mAb
Primary antibody: PKC beta Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 77 kDa
Observed MW: 77 kDa
免疫细胞化学
ICC shows positive staining in K562 cells (top panel) and negative staining in HCT116 cells (below panel). Anti-PKC beta antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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