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Rabbit抗原名称
Phospho-YAP (Ser127)分子别名
Transcriptional coactivator YAP1; Yes-associated protein 1; Protein yorkie homolog; Yes-associated protein YAP65 homolog; YAP65; YAP1细胞定位
Cytoplasm, Nucleus, Cell membraneAccession
P46937克隆号
S-4250抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu, Ms, Rt阳性样本
A431, PANC-1, Calyculin A treated HeLa, NIH/3T3, C2C12, C6纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu, Ms, Rt IHC-P 1:500 Hu, Ms, Rt
Phospho-YAP (Ser127) is a phosphorylated form of the Yes-associated protein (YAP), which is a key transcriptional coactivator in the Hippo signaling pathway. YAP regulates various cellular processes, including cell proliferation, survival, differentiation, and organ size. The phosphorylation of YAP at serine 127 by kinases such as MST1/2 and LATS1/2 leads to its inactivation and cytoplasmic retention. When dephosphorylated, YAP can translocate to the nucleus and promote gene transcription. For example, androgen exposure can suppress the inhibitory phospho-Ser127 modification on YAP, resulting in increased YAP nuclear abundance and activity. This regulation is crucial for understanding the role of YAP in diseases like cancer.
免疫印迹
WB result of Phospho-YAP (Ser127) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-YAP (Ser127) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A431 whole cell lysate 20 µg
Lane 2: PANC-1 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 70 kDa
This blot was developed with high sensitivity substrateWB result of Phospho-YAP (Ser127) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-YAP (Ser127) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 ng/ml Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 85 kDa
This blot was developed with high sensitivity substrateWB result of Phospho-YAP (Ser127) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-YAP (Ser127) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 70 kDa
This blot was developed with high sensitivity substrateWB result of Phospho-YAP (Ser127) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-YAP (Ser127) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 70 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human lung cancer. Anti- Phospho-YAP (Ser127) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti- Phospho-YAP (Ser127) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti- Phospho-YAP (Ser127) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti- Phospho-YAP (Ser127) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Phospho-YAP (Ser127) in tumor tissue.
Expression of Phospho-YAP (Ser127) in human tissue.
Expression of Phospho-YAP (Ser127) in mouse & rat tissue.
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