NOX2/gp91phox Recombinant Rabbit mAb (S-2974-75)
NADPH oxidase 2,CGD91-phox,Cytochrome b(558) subunit beta (Cytochrome b558 subunit beta),Cytochrome b-245 heavy chain,Heme-binding membrane glycoprotein gp91phox,Neutrophil cytochrome b 91 kDa polypeptide,CYBB
分享产品介绍 评论(0)
宿主来源
Rabbit抗原名称
NOX2/gp91phox分子别名
NADPH oxidase 2; CGD91-phox; Cytochrome b(558) subunit beta (Cytochrome b558 subunit beta); Cytochrome b-245 heavy chain; Heme-binding membrane glycoprotein gp91phox; Neutrophil cytochrome b 91 kDa polypeptide; CYBB免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
P04839克隆号
S-2974-75抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
稀释度
应用 稀释度 推荐种属 IHC-P 1:1000 Hu
NOX2/gp91phox, encoded by the CYBB gene on chromosome X, is a 570-amino-acid, ~91 kDa glycosylated flavohemoprotein that forms the catalytic core of the phagocyte NADPH oxidase complex; its N-terminal six-transmembrane α-helical domain anchors it to the plasma membrane, binds p22phox, and ligates two non-identical hemes that sequentially transfer electrons across the membrane, while its C-terminal cytoplasmic tail docks NADPH and FAD, enabling a four-step redox cascade that reduces extracellular O₂ to microbicidal superoxide (O₂⁻), thereby driving the respiratory burst essential for killing invading pathogens; genetic loss-of-function mutations in gp91phox cause chronic granulomatous disease, whereas its overactivation contributes to oxidative stress implicated in atherosclerosis, hepatic fibrosis, and other inflammatory disorders.
免疫组化
IHC shows positive staining in paraffin-embedded human spleen. Anti-NOX2/gp91phox antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-NOX2/gp91phox antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-NOX2/gp91phox antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-NOX2/gp91phox antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of NOX2/gp91phox in tumor tissue.
Expression of NOX2/gp91phox in human tissue.
评论(0)