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宿主来源
Rabbit抗原名称
MAP2分子别名
Microtubule-associated protein 2; MAP-2; Mtap2; Map2免疫原
Synthetic Peptide细胞定位
Cytoplasm, CytoskeletonAccession
P20357克隆号
S-3110-72抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms, Rt纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
IF ?
稀释度
应用 稀释度 推荐种属 IHC-P 1:250 Ms, Rt IF 1:100 Ms, Rt
MAP2 (microtubule-associated protein 2) is a ~280 kDa heat-stable phosphoprotein expressed almost exclusively in neurons, where it binds along the sides of microtubules in dendrites, stabilizing them and determining dendritic caliber and branching by spacing microtubules apart; its single tandemly repeated 31-amino-acid microtubule-binding domain is regulated by phosphorylation, and proteolytic cleavage of the projection domain during excitotoxic or ischemic insults produces 40–50 kDa fragments that rapidly depolymerize dendritic microtubules, contributing to neuronal death, while antibodies to these fragments serve as widely used selective markers of dendritic cytoarchitecture in neurohistochemistry.
免疫组化
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-MAP2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse kidney. Anti-MAP2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-MAP2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-MAP2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat cerebral cortex. Anti-MAP2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of MAP2 in mouse & rat tissue.
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