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Rabbit抗原名称
KIF18A分子别名
Kinesin-like protein KIF18A; Marrow stromal KIF18A (MS-KIF18A)免疫原
Synthetic Peptide细胞定位
Cytoplasm, Cytoskeleton, NucleusAccession
Q8NI77克隆号
S-3310-11抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HeLa, A549, Jurkat, MCF7, F9, Neuro-2a, raw264.7, rat testis, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk IHC-P 1:1000 Hu
KIF18A is a kinesin-8 family motor protein that uses ATP hydrolysis to move along microtubules, selectively accumulating at the plus ends of kinetochore fibers where it dampens their dynamics to ensure precise chromosome congression on the metaphase plate, control spindle length and tension, and coordinate subsequent cytokinesis, thereby safeguarding genomic integrity; its depletion triggers metaphase alignment errors, anaphase delay, central-spindle disorganization, and incomplete cleavage furrow formation, while its overexpression is strongly linked to aggressive cancers with chromosomal instability, making it a promising selective therapeutic target for small-molecule inhibitors that induce mitotic catastrophe preferentially in aneuploid tumor cells.
免疫印迹
WB result of KIF18A Recombinant Rabbit mAb
Primary antibody: KIF18A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 102 kDa
Observed MW: 102 kDaWB result of KIF18A Recombinant Rabbit mAb
Primary antibody: KIF18A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: F9 whole cell lysate 20 µg
Lane 2: Neuro-2a whole cell lysate 20 µg
Lane 3: raw264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 102 kDa
Observed MW: 102 kDaWB result of KIF18A Recombinant Rabbit mAb
Primary antibody: KIF18A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 102 kDa
Observed MW: 102 kDaWB result of KIF18A Recombinant Rabbit mAb
Primary antibody: KIF18A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 102 kDa
Observed MW: 102 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-KIF18A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-KIF18A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-KIF18A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of KIF18A in tumor tissue.
Expression of KIF18A in human tissue.
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