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宿主来源
Rabbit抗原名称
JAK3分子别名
Tyrosine-protein kinase JAK3; Janus kinase 3 (JAK-3); Leukocyte janus kinase (L-JAK); JAK3细胞定位
CytoplasmAccession
P52333克隆号
S-4219抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
Karpass-299, NK-92纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:500 Hu ICC 1:100 Hu
JAK3, which stands for Janus Kinase 3, is a non-receptor tyrosine kinase that plays a crucial role in the immune system. It is predominantly expressed in immune cells such as T cells, B cells, and natural killer cells. JAK3 is involved in the intracellular signaling pathways initiated by cytokine receptors, particularly those of the common gamma chain family, including interleukin-2 (IL-2), IL-4, IL-7, IL-9, IL-15, and IL-21 receptors. Upon receptor activation, JAK3 phosphorylates downstream signaling molecules, leading to the activation of transcription factors like STATs and ultimately regulating cell proliferation, differentiation, and survival. Mutations in the JAK3 gene can result in severe combined immunodeficiency (SCID), highlighting its essential function in immune development and function. Additionally, due to its central role in immune signaling, JAK3 has become a target for therapeutic interventions in autoimmune and inflammatory diseases.
免疫印迹
WB result of Jak3 Recombinant Rabbit mAb
Primary antibody: Jak3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Karpass-299 whole cell lysate 20 µg
Lane 2: NK-92 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 125 kDa
Observed MW: 115 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Jak3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma. Anti-Jak3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human NK/T-cell lymphoma. Anti-Jak3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in KARPAS-299 cells. Anti- Jak3 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of Jak3 in tumor tissue.
Expression of Jak3 in human tissue.
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