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Jak1 Recombinant Rabbit mAb (S-4201)

Tyrosine-protein kinase JAK1,Janus kinase 1 (JAK-1),JAK1A,JAK1B,JAK1

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6600
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    JAK1

  • 分子别名

    Tyrosine-protein kinase JAK1; Janus kinase 1 (JAK-1); JAK1A; JAK1B; JAK1

  • Accession

    P23458

  • 克隆号

    S-4201

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HDLM-2, K562, PC-3, DU 145, CTLL-2, PC-12

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms, Rt
    IHC-P 1:2000 Hu, Ms, Rt
背景介绍

  • Jak1 (Janus kinase 1) is a 130-kDa, membrane-proximal tyrosine kinase that associates with the cytoplasmic tails of cytokine receptors—such as those for interferons, interleukins and gp130 family ligands—and, upon ligand-driven receptor clustering, trans-autophosphorylates its activation loop and then phosphorylates STAT proteins to trigger rapid gene-expression programs governing immunity, hematopoiesis and growth; because its ATP-binding cleft can be occupied by ruxolitinib, upadacitinib or other clinical inhibitors, Jak1 is a validated drug target in autoimmune diseases, myeloproliferative neoplasms and alloimmune disorders.

  • 免疫印迹

    • WB result of Jak1 Recombinant Rabbit mAb
      Primary antibody: Jak1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: LNCaP whole cell lysate 20 µg
      Lane 2: 22RV1 whole cell lysate 20 µg
      Lane 3: HDLM-2 whole cell lysate 20 µg
      Lane 4: K562 whole cell lysate 20 µg
      Lane 5: PC-3 whole cell lysate 20 µg
      Lane 6: DU 145 whole cell lysate 20 µg
      Low expression control: LNCaP whole cell lysate; 22RV1 whole cell lysate
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 133 kDa
      Observed MW: 120 kDa

    • WB result of Jak1 Recombinant Rabbit mAb
      Primary antibody: Jak1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: CTLL-2 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 133 kDa
      Observed MW: 120 kDa

    • WB result of Jak1 Recombinant Rabbit mAb
      Primary antibody: Jak1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: PC-12 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 133 kDa
      Observed MW: 120 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Jak1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Jak1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Jak1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-Jak1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Jak1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-Jak1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 组织表达图谱

    • Expression of Jak1 in human tissue.

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