CHOP Recombinant Mouse mAb (S-3924)
DNA damage-inducible transcript 3 protein,DDIT-3,C/EBP zeta,C/EBP-homologous protein (CHOP),C/EBP-homologous protein 10 (CHOP-10),CCAAT/enhancer-binding protein homologous protein,Growth arrest and DNA damage-inducible protein GADD153,CHOP,CHOP10,GADD153,DDIT3
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宿主来源
Mouse抗原名称
CHOP分子别名
DNA damage-inducible transcript 3 protein; DDIT-3; C/EBP zeta; C/EBP-homologous protein (CHOP); C/EBP-homologous protein 10 (CHOP-10); CCAAT/enhancer-binding protein homologous protein; Growth arrest and DNA damage-inducible protein GADD153; CHOP; CHOP10; GADD153; DDIT3细胞定位
Cytoplasm, NucleusAccession
P35638克隆号
S-3924抗体类型
Mouse mAb抗体同种型
IgG2a反应种属 ?
Hu, Ms, Rt阳性样本
Tunicamycin treated HeLa纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ChIP
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu ICC 1:500 Hu ChIP 1:20-1:50 Ms
CHOP (C/EBP homologous protein) is a 29 kDa, 169-amino-acid bZIP transcription factor whose basic DNA-binding region carries two disruptive proline substitutions that enable it to form non-DNA-binding heterodimers with other C/EBP or ATF4 partners, thereby acting as a dominant-negative repressor of many C/EBP target genes while also partnering with ATF4 to transcriptionally activate pro-apoptotic genes such as GADD34, BIM, DR5 and TRB3, leading—after phosphorylation by p38 MAPK at Ser78/81 and JNK-mediated enhancement—to mitochondrial outer-membrane permeabilization, cytochrome-c release, caspase activation and endoplasmic-reticulum-stress-induced apoptosis.
免疫印迹
WB result of CHOP Recombinant Mouse mAb
Primary antibody: CHOP Recombinant Mouse mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 2 µg/ml tunicamycin for 8 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 30 kDa
This blot was developed with high sensitivity substrate
免疫细胞化学
ICC analysis of HeLa cells treated with tunicamycin (2μg/ml, 8h) (top panel) and untreated HeLa cells (below panel). Anti-CHOP antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
Chromatin immunoprecipitation (ChIP) was performed on MEF (left) / MEF + tunicamycin (2ug/ml, overnight) (+) (right) cross-linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used CHOP Recombinant Mouse mAb (S-3924) and Mouse mAb IgG1, κ Isotype Control (S-844-79) at 1:50 for immunoprecipitation.
Post-immunoprecipitation, both samples were washed, eluted, and cross-links reversed. Purified DNA was analyzed by qPCR.
qPCR showed the enrichment of Atf3 and Rpl30
in CHOP Recombinant Mouse mAb (S-3924)-
immunoprecipitated sample.
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