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宿主来源
Rabbit抗原名称
CD275分子别名
ICOS ligand; B7 homolog 2 (B7-H2); B7-like protein Gl50; B7-related protein 1 (B7RP-1); B7H2; B7RP1; ICOSL; KIAA0653; ICOSLG免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
O75144克隆号
S-3306-12抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
Raji, Daudi, HL-60, MCF7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu IHC-P 1:250 Hu
CD275, also called ICOS ligand (ICOSL) or B7-H2, is a 40–60 kDa glycosylated type-I transmembrane protein encoded by the ICOSLG gene on chromosome 21 and classified within the B7 and immunoglobulin superfamilies; it is expressed on B cells, dendritic cells, macrophages, monocytes, endothelial cells and various non-hematopoietic tissues, can be rapidly up-regulated by IFN-γ, TNF-α or LPS, and serves as the indispensable ligand for the inducible costimulator ICOS on activated T cells, thereby delivering a critical positive co-stimulatory signal that drives T-cell activation, proliferation, Th2 cytokine secretion and the generation of follicular helper T cells and germinal centers while simultaneously promoting B-cell differentiation into plasma cells and antibody production during secondary immune responses, and plays additional roles in reactivating effector/memory T cells on inflamed endothelium to control tissue entry and in skewing maternal immunity toward a protective Th2 phenotype during pregnancy; its dysregulation contributes to infection, hypersensitivity, autoimmune diseases, transplantation immunity and tumor immunity, and alternative splicing yields multiple isoforms with distinct tissue distributions and functions.
免疫印迹
WB result of B7-H2/ICOSLG Recombinant Rabbit mAb
Primary antibody: B7-H2/ICOSLG Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: Raji whole cell lysate 20 µg
Lane 3: Daudi whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Lane 5: HL-60 whole cell lysate 20 µg
Low expression control: Jurkat whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 40-80 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti- B7-H2/ICOSLG antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti- B7-H2/ICOSLG antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti- B7-H2/ICOSLG antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of B7-H2/ICOSLG in tumor tissue.
Expression of B7-H2/ICOSLG in human tissue.
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