Phospho-IKKα/β (Ser176/180) Recombinant Rabbit mAb (S-4059)
Inhibitor of nuclear factor kappa-B kinase subunit alpha,I-kappa-B kinase alpha,IKK-A,IKK-alpha,IkBKA,IkappaB kinase,Conserved helix-loop-helix ubiquitous kinase,I-kappa-B kinase 1 (IKK-1,IKK1),Nuclear factor NF-kappa-B inhibitor kinase alpha (NFKBIKA),Transcription factor 16 (TCF-16),IKKA,TCF16,CHUK
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宿主来源
Rabbit抗原名称
Phospho-IKKα/β (Ser176/180)分子别名
Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1 (IKK-1; IKK1); Nuclear factor NF-kappa-B inhibitor kinase alpha (NFKBIKA); Transcription factor 16 (TCF-16); IKKA; TCF16; CHUK细胞定位
Cytoplasm, NucleusAccession
O15111克隆号
S-4059抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu, Ms, Rt阳性样本
TNF-alpha and Calyculin A treated HeLa纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:500 Hu
Phospho-IKKα/β (Ser176/180) refers to the phosphorylated, catalytically active forms of the IκB kinase (IKK) catalytic subunits IKKα (at Ser176/Ser180) and IKKβ (at Ser177/Ser181); these activating phosphorylation events, induced by inflammatory cytokines such as TNF-α and IL-1β, trigger a conformational switch that fully activates the IKK complex, enabling it to phosphorylate the NF-κB inhibitor IκBα and thus liberate the transcription factor NF-κB for nuclear import and pro-inflammatory gene expression.
免疫印迹
WB result of Phospho-IKKα/β (Ser176/180) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-IKKα/β (Ser176/180) Recombinant Rabbit mAb at 1/1000 dilution
Primary antibody incubation conditions: overnight at 4°C
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 20 ng/ml TNF-alpha and 50 nM Calyculin A for 5 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 85 kDa
Observed MW: 90 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human breast cancer. Anti- Phospho-IKKα/β (Ser176/180) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti- Phospho-IKKα/β (Ser176/180) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti- Phospho-IKKα/β (Ser176/180) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti- Phospho-IKKα/β (Ser176/180) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Phospho-IKKα/β (Ser176/180) in tumor tissue.
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