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宿主来源
Rabbit抗原名称
VANGA2分子别名
Vang-like protein 2; Loop-tail protein 1 homolog; Strabismus 1; Van Gogh-like protein 2; KIAA1215; STB1; VANGL2免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
Q9ULK5克隆号
S-2938-231抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms, Rt阳性样本
NIH/3T3, mouse brain, rat brain纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt ICC 1:100 Ms
VANGL2 (Van Gogh-like 2) is a four-pass transmembrane core component of the Wnt/planar cell polarity (PCP) pathway that—by shuttling between the endoplasmic reticulum, plasma membrane and endocytic vesicles—governs embryonic convergence-extension, neural crest migration, adult tissue homeostasis and repair, while in disease contexts it drives malignant traits: in glioma it is transcriptionally up-regulated, correlates with IDH mutation and poor prognosis, and accelerates proliferation, invasion and stem-like sphere formation via positive crosstalk with Notch signaling, whereas in inflammatory disorders it unexpectedly suppresses NF-κB by acting as an autophagy adaptor that recruits the E3 ligase PDLIM2 to K63-ubiquitinate the p65 subunit, thereby enabling NDP52-mediated selective autophagic degradation of p65 and limiting pro-inflammatory cytokine output.
免疫印迹
WB result of VANGA2 Recombinant Rabbit mAb
Primary antibody: VANGA2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 62 kDaWB result of VANGA2 Recombinant Rabbit mAb
Primary antibody: VANGA2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 62 kDa
免疫细胞化学
ICC shows positive staining in NIH/3T3 cells. Anti- VANGA2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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