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Rabbit抗原名称
TDG分子别名
G/T mismatch-specific thymine DNA glycosylase; Thymine-DNA glycosylase (hTDG)免疫原
Synthetic Peptide细胞定位
NucleusAccession
Q13569克隆号
S-3111-16抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
293T, HeLa, K562, Jurkat, HCT 116纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:50 Hu
TDG (thymine DNA glycosylase) is a 410-residue nuclear enzyme that belongs to the uracil DNA glycosylase (UDG) superfamily and acts as a key guardian of epigenetic stability by excising thymine, 5-formylcytosine, 5-carboxylcytosine and other deaminated or oxidized bases from G•T, G•U or G•5fC/5caC mispairs in double-stranded DNA, thereby initiating base-excision repair (BER), reshaping CpG methylation landscapes and preventing C→T transition mutations and transcriptional misregulation in embryonic stem cells and somatic tissues.
免疫印迹
WB result of TDG Recombinant Rabbit mAb
Primary antibody: TDG Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: HCT 116 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 46 kDa
Observed MW: 50, 60 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-TDG antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-TDG antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-TDG antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-TDG antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-TDG antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of TDG in tumor tissue.
Expression of TDG in human tissue.
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