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宿主来源
Rabbit抗原名称
HSF1分子别名
Heat shock factor protein 1; HSF 1; Heat shock transcription factor 1Imported (HSTF 1); HSTF1免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
Q00613克隆号
S-3139-44抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
PC-3, HeLa, K562, NIH/3T3纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:1000 Hu ICC 1:500 Hu, Ms IF 1:500 Hu
Heat shock factor 1 (HSF1) is an evolutionarily conserved transcription factor that acts as the master regulator of the inducible heat-shock response by binding to heat-shock elements (HSEs) in the promoters of cytoprotective chaperone genes such as HSP70 and HSP90, thereby driving rapid expression of molecular chaperones that prevent stress-induced protein misfolding and aggregation; beyond this canonical cytoprotective role, HSF1 also modulates diverse cellular processes including development, metabolism, apoptosis and aging, is frequently hijacked by cancers to sustain oncogenic protein homeostasis and promote malignant proliferation, and is therefore emerging as a key therapeutic target in both protein-misfolding disorders and cancer.
免疫印迹
WB result of HSF1 Recombinant Rabbit mAb
Primary antibody: HSF1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-3 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 80 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-HSF1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-HSF1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-HSF1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-HSF1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti-HSF1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
免疫荧光
IF shows positive staining in paraffin-embedded human ovarian cancer. Anti-HSF1 antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 647) (S0B4005) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of HSF1 in tumor tissue.
Expression of HSF1 in human tissue.
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