分享产品介绍 评论(0)
宿主来源
Rabbit抗原名称
ATF1分子别名
Cyclic AMP-dependent transcription factor ATF-1; cAMP-dependent transcription factor ATF-1; Activating transcription factor 1; TCR-ATF1; Atf1免疫原
Synthetic Peptide细胞定位
NucleusAccession
P81269克隆号
S-2883-124抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
HeLa, 293T, A431, K562, RAW264.7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms ICC 1:500 Hu, Ms
ATF1 (activating transcription factor 1) is a 271-amino-acid basic leucine-zipper (bZIP) protein that forms homo- or heterodimers with c-Jun, CREB or other bZIP partners to bind the consensus cAMP response element 5′-TGACGTCA-3′, thereby functioning as a stimulus-inducible transcriptional activator in cAMP, MAPK, calcium and stress-signaling cascades that govern proliferation, survival, melanocyte differentiation, synaptic plasticity and memory; it is subject to phosphorylation-driven control (notably by PKA at Ser63 and by p38/MSK1 at Ser63/121), acetylation and ubiquitination, is frequently fused to either EWSR1 or FUS in Ewing sarcoma and angiomatoid fibrous histiocytoma through t(12;22) or t(12;16) chromosomal translocations that convert it into an aberrant transcriptional activator, and its locus at 12q13 is co-amplified with CDK4 in a variety of sarcomas, underscoring its dual role as both a tightly regulated physiological transcription factor and an oncogenic driver.
免疫印迹
WB result of ATF1 Recombinant Rabbit mAb
Primary antibody: ATF1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 37 kDa
This blot was developed with high sensitivity substrateWB result of ATF1 Recombinant Rabbit mAb
Primary antibody: ATF1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 37 kDa
This blot was developed with high sensitivity substrate
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- ATF1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in RAW264.7 cells. Anti-ATF1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
评论(0)