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Rabbit抗原名称
SP1分子别名
Transcription factor Sp1; TSFP1细胞定位
Cytoplasm, NucleusAccession
P08047克隆号
S-4102抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Mk阳性样本
HeLa, 293T, Ramos, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ChIP
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Mk IHC-P 1:2000 Hu ICC 1:500 Hu ChIP 1:20-1:50 Hu
SP1 (specificity protein 1) is a ubiquitous, C2H2-type zinc-finger transcription factor that binds GC-rich motifs (GGGCGG and related sequences) in the promoters and enhancers of thousands of human genes via its three C-terminal zinc fingers, recruiting TFIID, TFIIB, histone acetyl-transferases (p300/CBP, PCAF) and chromatin-remodeling complexes to activate or fine-tune basal and signal-dependent transcription of housekeeping, metabolic, cell-cycle, angiogenic and differentiation genes; it is tightly regulated through phosphorylation by multiple kinases (ERK, AKT, DNA-PK, CDK), acetylation, SUMOylation, prolyl-isomerization and redox status of its two critical cysteines, and its activity is modulated by oncogenic, nutrient and stress signaling, making SP1 a central hub in cancer, diabetes, cardiovascular and neurodegenerative diseases, with elevated expression often correlating with poor prognosis, while synthetic mithramycin, chromomycin and novel zinc-finger inhibitors are explored to target its DNA-binding or protein–protein interfaces therapeutically.
免疫印迹
WB result of SP1 Recombinant Rabbit mAb
Primary antibody: SP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: Ramos whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 81 kDa
Observed MW: 100 kDaWB result of SP1 Recombinant Rabbit mAb
Primary antibody: SP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 81 kDa
Observed MW: 100 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-SP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-SP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-SP1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used SP1 Recombinant Rabbit mAb (S-4102) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
Post -immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
qPCR showed the enrichment of DHFR, TNIP1 and SAT-α in SP1 Recombinant
Rabbit mAb (S-4102) -immunoprecipitated sample.
组织表达图谱
Expression of SP1 in tumor tissue.
Expression of SP1 in human tissue.
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