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Phospho-SMAD1/5 (Ser463/465) Recombinant Rabbit mAb (S-4058)

Mothers against decapentaplegic homolog 5,MAD homolog 5,Mothers against DPP homolog 5,JV5-1,SMAD family member 5 (SMAD 5,Smad5,hSmad5),MADH5,Mothers against decapentaplegic homolog 1,MAD homolog 1,Mothers against DPP homolog 1,JV4-1,Mad-related protein 1,SMAD family member 1 (SMAD 1,Smad1,hSMAD1),Transforming growth factor-beta-signaling protein 1 (BSP-1),BSP1,MADH1,MADR1

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6577
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Phospho-SMAD1 (Ser463/465)/ SMAD5 (Ser463/465)

  • 分子别名

    Mothers against decapentaplegic homolog 5; MAD homolog 5; Mothers against DPP homolog 5; JV5-1; SMAD family member 5 (SMAD 5; Smad5; hSmad5); MADH5; Mothers against decapentaplegic homolog 1; MAD homolog 1; Mothers against DPP homolog 1; JV4-1; Mad-related protein 1; SMAD family member 1 (SMAD 1; Smad1; hSMAD1); Transforming growth factor-beta-signaling protein 1 (BSP-1); BSP1; MADH1; MADR1

  • 细胞定位

    Nucleus, Mitochondrion, Cytoplasm

  • Accession

    Q99717, Q15797

  • 克隆号

    S-4058

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 翻译后修饰类型

    磷酸化

  • 反应种属 ?

    Hu, Ms

  • 阳性样本

    Human BMP2 treated HepG2 cells, Human BMP2 treated MEF cells

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms
背景介绍

  • Phospho-SMAD1/5 (Ser463/465) is a key activated transcriptional regulator in the Bone Morphogenetic Protein (BMP) signaling pathway. When BMP ligands bind to type I and type II serine/threonine kinase receptors on the cell membrane, the activated receptor complex specifically phosphorylates the serine residues at position 463 (in SMAD1) and 465 (in SMAD5) within the C-terminal SXS motif of SMAD1 or SMAD5 proteins. This phosphorylation event is the central step in SMAD1/5 activation, prompting them to form a heterotrimeric complex with the common mediator SMAD4 and translocate into the nucleus. There, they regulate the transcription of downstream target genes (such as Id1, Id2, etc.), mediating the crucial role of BMP signaling in various biological processes including cell proliferation, differentiation, apoptosis, as well as embryonic development, bone formation, and homeostasis maintenance. The phosphorylation level at this site serves as a classic molecular marker for assessing the activity of the BMP/SMAD pathway.

  • 免疫印迹

    • WB result of Phospho-SMAD1/5 (Ser463/465) Recombinant Rabbit mAb
      Blocking/Diluting buffer and concentration: 5% NFDM/TBST
      Primary antibody: Phospho-SMAD1/5 (Ser463/465) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated HepG2 whole cell lysate 20 µg
      Lane 2: HepG2 treated with 50 ng/ml Human BMP2 for 30 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 52 kDa
      Observed MW: 60 kDa

    • WB result of Phospho-SMAD1/5 (Ser463/465) Recombinant Rabbit mAb
      Blocking/Diluting buffer and concentration: 5% NFDM/TBST
      Primary antibody: Phospho-SMAD1/5 (Ser463/465) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated MEF whole cell lysate 20 µg
      Lane 2: MEF treated with 50 ng/ml Human BMP2 for 30 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 52 kDa
      Observed MW: 60 kDa

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