分享产品介绍 评论(0)
宿主来源
Rabbit抗原名称
SLC12A2分子别名
Solute carrier family 12 member 2; Basolateral Na-K-Cl symporter; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2 (BSC2); Na-K-2Cl cotransporter 1 (hNKCC1); NKCC1 s免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
P55011克隆号
S-2437-30抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
MCF7, HT-29, COLO 205, NIH/3T3, mouse brain纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms, Rt IHC-P 1:200 Hu, Ms
SLC12A2 (solute carrier family 12 member 2), also known as NKCC1 (Na⁺-K⁺-2Cl⁻ cotransporter-1), is a 1,215-residue, bumetanide-sensitive, electromeutral plasma-membrane protein that uses the inward Na⁺ gradient generated by Na⁺/K⁺-ATPase to drive uphill import of K⁺ and Cl⁻ into cells, thereby regulating ionic homeostasis, cell volume, epithelial salt movement, blood pressure, hearing, GABAergic inhibition, and neuronal excitability through dynamic phosphorylation by oxidative-stress-responsive kinases (e.g., SPAK/OSR1) and membrane trafficking in epithelia, neurons, vascular smooth muscle, immune cells, and secretory glands, with pathophysiological relevance to hypertension, deafness, neuropathic pain, and tumor cell proliferation.
免疫印迹
WB result of SLC12A2 Recombinant Rabbit mAb
Primary antibody: SLC12A2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: HT-29 whole cell lysate 20 µg
Lane 3: COLO 205 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 131 kDa
Observed MW: 120-220 kDaWB result of SLC12A2 Recombinant Rabbit mAb
Primary antibody: SLC12A2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Negative control: mouse liver lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 131 kDa
Observed MW: 180-220 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-SLC12A2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-SLC12A2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-SLC12A2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of SLC12A2 in tumor tissue.
Expression of SLC12A2 in human tissue.
Expression of SLC12A2 in mouse & rat tissue.
评论(0)