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Rabbit抗原名称
Siglec-8分子别名
Sialic acid-binding Ig-like lectin 8; Sialoadhesin family member 2 (SAF-2); SAF2; SIGLEC8免疫原
Synthetic Peptide细胞定位
MembraneAccession
Q9NYZ4克隆号
S-2049-19抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:5000 Hu ICC 1:500 Hu
Siglec-8 is a CD33-related cell-surface lectin selectively expressed on human eosinophils and, at lower density, on mast cells and basophils; it carries an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an ITIM-like motif that, upon engagement by sialylated glycan ligands, recruit SHP-1/2 phosphatases to deliver a potent inhibitory signal that suppresses FcεRI- or cytokine-driven activation, survival and mediator release, thereby serving as a key “off-switch” for allergic inflammation and making it an attractive therapeutic target for eosinophilic asthma, chronic urticaria and other IgE-mediated diseases.
免疫印迹
WB result of Siglec-8 Recombinant Rabbit mAb
Primary antibody: Siglec-8 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector (vector control) containing a myc-His-tag whole cell Lysate 20 µg
Lane 2: 293T cells transfected with a human SIGLEC8 expression vector containing a myc-His-tag whole cell Lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 62 kDa
免疫细胞化学
ICC shows positive staining in Myc-His transfected 293F cells (top panel) and negative staining in vector-transfected 293F cells (below panel). Anti- Siglec-8 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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