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宿主来源
Rabbit抗原名称
SHARPIN分子别名
Sharpin; Shank-associated RH domain-interacting protein; Shank-interacting protein-like 1 (hSIPL1); SIPL1免疫原
Synthetic Peptide细胞定位
Cytoplasm, SynapseAccession
Q9H0F6克隆号
S-2356-308抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
PC-3, MCF7, T-47D, Raji, U-2 OS, A20, mouse brain, rat testis, COS-7预测反应种属
(反应种属缩写表)Bv纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk IHC-P 1:200 Hu ICC 1:500 Hu
SHARPIN (Shank-associated RH domain-interacting protein) is a ~34 kDa ubiquitin-like adaptor that forms the LUBAC complex with HOIP and HOIL-1L to catalyze M1-linked linear ubiquitination of NEMO and other substrates, thereby activating canonical NF-κB signaling, suppressing TNF-α-induced apoptosis, modulating integrin activation, stabilizing β-catenin and ERα to drive Wnt/estrogen-dependent transcription, inhibiting ferroptosis via the p53/SLC7A11/GPX4 axis, promoting Rap1-mediated melanoma metastasis, and regulating Aβ phagocytosis and NLRP3 inflammasome activity in macrophages, with its dysregulation linked to chronic dermatitis, autoimmunity, and cancers of breast, stomach, and skin.
免疫印迹
WB result of SHARPIN Recombinant Rabbit mAb
Primary antibody: SHARPIN Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-3 whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: T-47D whole cell lysate 20 µg
Lane 4: Raji whole cell lysate 20 µg
Lane 5: U-2 OS whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 38 kDaWB result of SHARPIN Recombinant Rabbit mAb
Primary antibody: SHARPIN Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A20 whole cell lysate 20 µg
Lane 2: mouse brian lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 38 kDaWB result of SHARPIN Recombinant Rabbit mAb
Primary antibody: SHARPIN Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 38 kDaWB result of SHARPIN Recombinant Rabbit mAb
Primary antibody: SHARPIN Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 38 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-SHARPIN antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-SHARPIN antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in PC-3 cells. Anti-SHARPIN antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of SHARPIN in tumor tissue.
Expression of SHARPIN in human tissue.
Expression of SHARPIN in mouse & rat tissue.
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