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宿主来源
Rabbit抗原名称
SFRP1分子别名
Secreted frizzled-related protein 1; FRP-1; sFRP-1; Secreted apoptosis-related protein 2 (SARP-2); FRP; FRP1; SARP2免疫原
Synthetic Peptide细胞定位
SecretedAccession
Q8N474克隆号
S-3071-18抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
A549, SK-MEL-28纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:200 Hu
SFRP1 (Secreted Frizzled-Related Protein 1) is a 313-amino-acid, cysteine-rich antagonist of Wnt signaling that is secreted into the extracellular space where it binds Wnt ligands and Frizzled receptors to modulate β-catenin–dependent and –independent pathways, thereby regulating embryonic segmentation, eye, heart and bone development, as well as adult tissue homeostasis, while its epigenetic or mutational silencing in cancers removes this brake on Wnt signaling and drives tumor progression, invasion and metastasis.
免疫印迹
WB result of SFRP1 Recombinant Rabbit mAb
Primary antibody: SFRP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: SK-MEL-28 whole cell lysate 20 µg
Lane 4: HEK-293 whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate
Low expression control: HEK-293 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 35 kDa
Observed MW: 35 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-SFRP1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-SFRP1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-SFRP1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-SFRP1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of SFRP1 in tumor tissue.
Expression of SFRP1 in human tissue.
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