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PFKL+PFKM Recombinant Rabbit mAb (S-1302-31)

ATP-dependent 6-phosphofructokinase,liver type,ATP-PFK,PFK-L,6-phosphofructokinase type B,Phosphofructo-1-kinase isozyme B (PFK-B),Phosphohexokinase,muscle type,PFK-M,6-phosphofructokinase type A,Phosphofructo-1-kinase isozyme A (PFK-A),PFKX

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6571
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    PFKL+PFKM

  • 分子别名

    ATP-dependent 6-phosphofructokinase, liver type; ATP-PFK; PFK-L; 6-phosphofructokinase type B; Phosphofructo-1-kinase isozyme B (PFK-B); Phosphohexokinase; ATP-dependent 6-phosphofructokinase, muscle type; PFK-M; 6-phosphofructokinase type A; Phosphofructo-1-kinase isozyme A (PFK-A); Phosphohexokinase; PFKX

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm

  • Accession

    P17858, P08237

  • 克隆号

    S-1302-31

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HeLa, A431, SK-BR-3, HCT 116, RAW264.7, NIH/3T3, C6, PC-12

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000-1:2000 Hu, Ms, Rt
    IHC-P 1:2000 Hu, Ms, Rt
    ICC 1:500 Hu
背景介绍

  • PFKL and PFKM are tissue-specific isoforms of phosphofructokinase-1 (PFK-1) that catalyze the committed step of glycolysis by phosphorylating fructose-6-phosphate to fructose-1,6-bisphosphate; PFKL (≈83 kDa) forms homotetrameric L4 complexes most abundant in liver, whereas PFKM (≈85 kDa) forms homotetrameric M4 complexes predominant in muscle, yet both can co-assemble into heterotetramers (e.g., M3L, M2L2, ML3) in cells such as erythrocytes, enabling finely tuned allosteric regulation by metabolites (activated by ADP, AMP, fructose-2,6-bisphosphate; inhibited by ATP, citrate) and post-translational modifications (phosphorylation, GlcNAcylation, S-nitrosylation, ubiquitination) that integrate nutritional and energy stress signals to control glucose flux, NADPH balance, and downstream biosynthetic pathways critical for systemic glucose homeostasis, redox buffering, and cancer metabolic reprogramming.

  • 免疫印迹

    • WB result of PFKL+PFKM Recombinant Rabbit mAb
      Primary antibody: PFKL+PFKM Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: A431 whole cell lysate 20 µg
      Lane 3: SK-BR-3 whole cell lysate 20 µg
      Lane 4: HCT 116 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 85 kDa
      Observed MW: 85 kDa

    • WB result of PFKL+PFKM Recombinant Rabbit mAb
      Primary antibody: PFKL+PFKM Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: RAW264.7 whole cell lysate 20 µg
      Lane 2: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 85 kDa
      Observed MW: 85 kDa

    • WB result of PFKL+PFKM Recombinant Rabbit mAb
      Primary antibody: PFKL+PFKM Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Lane 2: PC-12 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 85 kDa
      Observed MW: 85 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human colon. Anti-PFKL+PFKM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human liver. Anti-PFKL+PFKM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human testis. Anti-PFKL+PFKM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-PFKL+PFKM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-PFKL+PFKM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse colon. Anti-PFKL+PFKM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-PFKL+PFKM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat colon. Anti-PFKL+PFKM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-PFKL+PFKM antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of PFKL+PFKM in tumor tissue.

    • Expression of PFKL+PFKM in human tissue.

    • Expression of PFKL+PFKM in mouse & rat tissue.

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