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PERK Recombinant Rabbit mAb (S-2822-52)

Eukaryotic translation initiation factor 2-alpha kinase 3,PRKR-like endoplasmic reticulum kinase,Pancreatic eIF2-alpha kinase (HsPEK),Protein tyrosine kinase EIF2AK3,PEK,EIF2AK3

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6570
规格
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    PERK

  • 分子别名

    Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase (HsPEK); Protein tyrosine kinase EIF2AK3; PEK; EIF2AK3

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Endoplasmic reticulum

  • Accession

    Q9NZJ5

  • 克隆号

    S-2822-52

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms

  • 阳性样本

    MCF7, 293T, RD, K562, RAW264.7, NIH/3T3

  • 预测反应种属
    (反应种属缩写表)

    Bv

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms
背景介绍

  • PERK (protein kinase RNA-like endoplasmic reticulum kinase) is an ER-resident transmembrane serine/threonine kinase that, under ER stress caused by misfolded protein accumulation, oligomerizes and auto-phosphorylates to phosphorylate eIF2α, transiently attenuating global cap-dependent translation while selectively up-regulating ATF4 and other stress-response mRNAs, thereby activating a transcriptional program that enhances folding capacity, antioxidant responses, and autophagy, and if stress is unresolved triggers apoptosis, making PERK a central regulator of the unfolded protein response, cancer cell survival, metabolic homeostasis, and neurodegenerative diseases.

  • 免疫印迹

    • WB result of PERK Recombinant Rabbit mAb
      Primary antibody: PERK Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: MCF7 whole cell lysate 20 µg
      Lane 2: 293T whole cell lysate 20 µg
      Lane 3: RD whole cell lysate 20 µg
      Lane 4: K562 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 125 kDa
      Observed MW: 140 kDa
      This blot was developed with high sensitivity substrate

    • WB result of PERK Recombinant Rabbit mAb
      Primary antibody: PERK Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: RAW264.7 whole cell lysate 20 µg
      Lane 2: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 125 kDa
      Observed MW: 140 kDa
      This blot was developed with high sensitivity substrate

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