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Rabbit抗原名称
NINJ1分子别名
Ninjurin-1; hNINJ1; Nerve injury-induced protein 1免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
Q92982克隆号
S-3109-56抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Mk阳性样本
HepG2, A549, HeLa, Jurkat, K562, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Mk IHC-P 1:1000 Hu
NINJ1, a 16 kDa evolutionary-conserved double-transmembrane protein first discovered as a nerve-injury-induced adhesion molecule, has emerged as the key executioner of plasma-membrane rupture (PMR) downstream of all major lytic cell-death pathways (pyroptosis, apoptosis, necroptosis, ferroptosis, PANoptosis, cuproptosis and parthanatos); upon death stimuli it oligomerizes into amphipathic filaments or “cookie-cutter” rings that breach the lipid bilayer, releasing damage-associated molecular patterns and propagating inflammation, while also regulating leukocyte trafficking, TLR4 signaling and blood–brain-barrier integrity, making it a promising therapeutic target in ischemia, sepsis, neuroinflammation and cancer.
免疫印迹
WB result of NINJ1 Recombinant Rabbit mAb
Primary antibody: NINJ1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 18 kDaWB result of NINJ1 Recombinant Rabbit mAb
Primary antibody: NINJ1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 18 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-NINJ1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-NINJ1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-NINJ1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-NINJ1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of NINJ1 in tumor tissue.
Expression of NINJ1 in human tissue.
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