产品中心 蛋白翻译后修饰(PTM)抗体 位点特异性修饰抗体
分享产品介绍 评论(0)
宿主来源
Rabbit抗原名称
NF-kB p65 (acetyl K310)分子别名
Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; NFKB3; RELA免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
Q04206克隆号
S-1113-161抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
乙酰化反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu
NF-kB p65 (acetyl K310) is the acetylated form of the p65 (RelA) subunit of the NF-κB transcription factor complex, wherein lysine 310 is acetylated primarily by the histone acetyltransferases CBP/p300, a modification that boosts its transcriptional activity without enhancing DNA binding or weakening IκB association; this acetylation, often preceded by phosphorylation at Ser 536 or Ser 276, recruits the bromodomain protein BRD4 to amplify expression of NF-κB-dependent inflammatory and immune genes, is reversed by HDAC3 and SIRT2 deacetylases to terminate signaling, and is dynamically regulated in physiology and diseases such as cancer, chronic inflammation and infection.
免疫印迹
WB result of NF-kB p65 (acetyl K310) Recombinant Rabbit mAb
Primary antibody: NF-kB p65 (acetyl K310) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T transfected with NF-kB p65 -myc-His fusion protein whole cell lysate 20 µg
Lane 2: 293T transfected with NF-kB p65-myc-His fusion protein and P300 (aa1287-1663)-myc-His fusion protein whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 70 kDa
评论(0)