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宿主来源
Rabbit抗原名称
Lysozyme分子别名
Lysozyme C; 1,4-beta-N-acetylmuramidase C; LZM; LYZ免疫原
Synthetic Peptide细胞定位
SecretedAccession
P61626抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
HL-60, THP-1, J774A.1纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms IHC-P 1:1000 Hu, Ms
Lysozyme is a small, ubiquitous enzyme that catalyzes the hydrolysis of β-1,4-glycosidic linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine in bacterial peptidoglycan, thereby weakening the cell wall and leading to osmotic lysis; it is abundant in tears, saliva, egg white, and other secretions, serving as a front-line innate immune defense against Gram-positive bacteria, and has been extensively studied since Alexander Fleming’s isolation in 1922, becoming a model system for protein folding, enzyme kinetics, and X-ray crystallography while finding applications in food preservation, pharmaceuticals, and biotechnology.
免疫印迹
WB result of Lysozyme Rabbit pAb
Primary antibody: Lysozyme Rabbit pAb at 1/1000 dilution
Lane 1: HL-60 whole cell lysate 20 µg
Lane 2: THP-1 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 15 kDaWB result of Lysozyme Rabbit pAb
Primary antibody: Lysozyme Rabbit pAb at 1/1000 dilution
Lane 1: J774A.1 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 15 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human small intestine. Anti-Lysozyme antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Lysozyme antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-Lysozyme antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Lysozyme antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Lysozyme antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Lysozyme in tumor tissue.
Expression of Lysozyme in human tissue.
Expression of Lysozyme in mouse & rat tissue.
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