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宿主来源
Rabbit抗原名称
GRIA2分子别名
Glutamate receptor 2; GluR-2; AMPA-selective glutamate receptor 2; GluR-B; GluR-K2; Glutamate receptor ionotropic; AMPA 2; GluA2; GLUR2免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P42262抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Ms, Rt阳性样本
mouse brain, rat brain预测反应种属
(反应种属缩写表)Or, CyMk纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:500 Ms IF 1:100 Ms, Rt
GRIA2 (Glutamate Ionotropic Receptor AMPA Type Subunit 2, formerly GluA2) is a 96-kDa, 883-aa transmembrane protein that assembles with other AMPA-receptor subunits to form calcium-impermeable, fast-desensitizing glutamate-gated cation channels critical for excitatory synaptic transmission and plasticity throughout the CNS; its unique Q/R-edited pore loop dictates low Ca²⁺ permeability, its C-tail binds PDZ scaffolds (GRIP, PICK1, NSF) to regulate surface trafficking and long-term potentiation/depression, and mutations or altered expression are linked to epilepsy, ischemic stroke damage, schizophrenia, Alzheimer’s disease and amyotrophic lateral sclerosis, making GRIA2 a key determinant of synaptic strength and neuronal survival.
免疫印迹
WB result of GRIA2 Rabbit pAb
Primary antibody: GRIA2 Rabbit pAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 99 kDa
Observed MW: 105 kDaWB result of GRIA2 Rabbit pAb
Primary antibody: GRIA2 Rabbit pAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 99 kDa
Observed MW: 105 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-GRIA2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse stomach. Anti-GRIA2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse brain. Anti- GRIA2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat brain. Anti- GRIA2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of GRIA2 in mouse & rat tissue.
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