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宿主来源
Rabbit抗原名称
GPHN分子别名
Gephyrin; GPH; KIAA1385免疫原
Synthetic Peptide细胞定位
Cell membrane, Cell projection, CytoplasmAccession
Q9NQX3克隆号
S-3167-63抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HEK-293, SH-SY5Y, MCF7, U-2 OS, Jurkat, RAW264.7, mouse testis, mouse brain, mouse heart, PC-12, C6, rat testis, rat brain纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:2000 Hu, Ms, Rt
GPHN (gephyrin) is a 93-kDa multifunctional scaffold protein that self-assembles into a hexagonal lattice beneath the postsynaptic membrane of inhibitory synapses, where it simultaneously anchors both glycine and GABA type A receptors to subsynaptic microtubules and actin cytoskeleton, thereby clustering and immobilizing these receptors to ensure reliable inhibitory neurotransmission, while also possessing dual enzymatic activities (molybdopterin adenylyltransferase and molybdotransferase) essential for the biosynthesis of the molybdenum cofactor, and its alternative splicing, extensive post-translational modifications and dynamic exchange at synapses modulate inhibitory synaptic strength and plasticity, with mutations or altered expression of GPHN linked to epilepsy, autism spectrum disorders, schizophrenia and molybdenum cofactor deficiency.
免疫印迹
WB result of GPHN Recombinant Rabbit mAb
Primary antibody: GPHN Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: SH-SY5Y whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: U-2 OS whole cell lysate 20 µg
Lane 5: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 80 kDa
Observed MW: 90 kDaWB result of GPHN Recombinant Rabbit mAb
Primary antibody: GPHN Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Lane 4: mouse heart lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 80 kDa
Observed MW: 90 kDaWB result of GPHN Recombinant Rabbit mAb
Primary antibody: GPHN Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Lane 3: rat testis lysate 20 µg
Lane 4: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 80 kDa
Observed MW: 90 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-GPHN antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-GPHN antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-GPHN antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-GPHN antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-GPHN antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-GPHN antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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