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Rabbit抗原名称
G3BP2分子别名
Ras GTPase-activating protein-binding protein 2; G3BP-2; GAP SH3 domain-binding protein 2; KIAA0660免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
Q9UN86克隆号
S-2386-66抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
293T, HeLa, Jurkat, A549, C2C12, mouse brain, C6, rat brain, COS-7预测反应种属
(反应种属缩写表)Or纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk
G3BP2 (Ras-GTPase-activating protein-binding protein 2) is a multifunctional RNA-binding scaffold protein that, through its NTF2, acidic, proline-rich, RRM and RGG domains, nucleates stress-granule assembly by forming homo- and hetero-oligomers with G3BP1, sequestering untranslated mRNPs in the cytoplasm during stress, while also regulating RNA stability (e.g., CDK7, CDK9, c-Myc), controlling nuclear-cytoplasmic shuttling of transcription factors such as NF-κB, TWIST1 and p53, and thereby influencing cell proliferation, epithelial-mesenchymal transition, viral infection, lipogenesis and neuronal viability in a context-dependent manner.
免疫印迹
WB result of G3BP2 Recombinant Rabbit mAb
Primary antibody: G3BP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 80 kDa
This blot was developed with high sensitivity substrateWB result of G3BP2 Recombinant Rabbit mAb
Primary antibody: G3BP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 80 kDa
This blot was developed with high sensitivity substrateWB result of G3BP2 Recombinant Rabbit mAb
Primary antibody: G3BP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 80 kDa
This blot was developed with high sensitivity substrateWB result of G3BP2 Recombinant Rabbit mAb
Primary antibody: G3BP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 80 kDa
This blot was developed with high sensitivity substrate
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