产品中心 抗体 其他一抗

所有图片(1/1)

Dinitrophenyl (DNP) Rabbit Polyclonal Antibody

价格 400.00 供应商现货 : 3-5个工作日
货号 S0B6559
规格
数量
收藏 分享

产品介绍 评论(0)

产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    Dinitrophenyl
  • 抗体类型

    Polyclonal antibody
  • 抗体同种型

    IgG
  • 纯化方式

    Immunogen Affinity
  • 浓度

    6 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS pH7.4, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt, 2 to 8 °C as supplied

  • 应用

    ELISA

背景介绍
  • Dinitrophenyl (DNP) is an important functional group in organic chemistry, consisting of a phenyl ring substituted with two nitro groups (-NO₂). The most common isomer is 2,4-dinitrophenyl, which is widely used due to its high reactivity and unique properties. In biochemistry and immunology, DNP is frequently employed as a hapten for antibody production or to study immune responses. In organic synthesis, it serves as a protecting group or a labeling moiety because the strong electron-withdrawing effect of the nitro groups makes the attached phenyl ring highly susceptible to nucleophilic substitution reactions. Additionally, DNP-containing compounds are often yellow and exhibit fluorescent properties, facilitating detection and analysis. It is important to note that DNP compounds, such as 2,4-dinitrophenol, were once used as weight-loss drugs or industrial dyes but are now strictly regulated due to their ability to disrupt cellular energy metabolism and pose a significant risk of causing hyperthermia.

  • ELISA

    • ELISA binding of Dinitrophenyl (DNP) Rabbit pAb with DNP-BSA conjugate. While the antibody does not recognize the BSA control.
      Coating antigen: DNP-BSA/BSA with 0.5 µg/ml.
      Dinitrophenyl (DNP) Rabbit pAb dilutions start from 1 µg/ml.
      EC50=10.63ng/ml.

评论(0)