分享产品介绍 评论(0)
宿主来源
Rabbit抗原名称
BSCL2/Seipin分子别名
Bernardinelli-Seip congenital lipodystrophy type 2 protein免疫原
Synthetic Peptide细胞定位
Endoplasmic reticulumAccession
Q96G97克隆号
S-1669-135抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HEK-293, Raji, HeLa, MCF7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:200 Hu
BSCL2/Seipin is an endoplasmic-reticulum-anchored multi-pass transmembrane protein that drives proper lipid-droplet biogenesis by catalyzing the clustering and directed budding of nascent neutral-lipid lenses within the ER membrane, thereby acting as a molecular scaffold that recruits and concentrates diacylglycerol acyltransferase, phosphatidic-acid-metabolizing enzymes and lipin-1 to nascent droplet formation sites, while missense mutations that disrupt its N-glycosylation motif trigger ER stress, ubiquitin–proteasome degradation and conformational seipinopathy leading to congenital generalized lipodystrophy type 2 or dominant motor-neuron degenerations.
免疫印迹
WB result of BSCL2/Seipin Recombinant Rabbit mAb
Primary antibody: BSCL2/Seipin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: U-2 OS whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: Raji whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Lane 5: MCF7 whole cell lysate 20 µg
Low expression control: U-2 OS whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 48 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human spinal cord. Anti-BSCL2/Seipin antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of BSCL2/Seipin in tumor tissue.
评论(0)