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Rabbit抗原名称
ARIH1分子别名
E3 ubiquitin-protein ligase ARIH1; H7-AP2; HHARI; Monocyte protein 6 (MOP-6); Protein ariadne-1 homolog (ARI-1); UbcH7-binding protein; ARI; MOP6; UBCH7BP免疫原
Synthetic Peptide细胞定位
Nucleus, CytoplasmAccession
Q9Y4X5克隆号
S-3166-64抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HeLa, A431, MCF7, PC-3, NIH/3T3, C6, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk ICC 1:500 Hu, Ms
ARIH1 (Ariadne RBR E3 ubiquitin-protein ligase 1) is a 557-amino-acid RING-between-RING (RBR) E3 ligase that ubiquitinates multiple substrates—most notably DNA-PKcs, whose proteasomal degradation activates the cGAS-STING pathway to enhance anti-tumor immunity, while in colorectal cancer it instead promotes K63-linked ubiquitination of PHB1 to drive mitochondrial respiration and tumor progression—thereby acting as a context-dependent regulator of proliferation, DNA repair, epithelial-mesenchymal transition and immune evasion through its dynamic interactions with E2 enzymes and cullin-RING ligase complexes.
免疫印迹
WB result of ARIH1 Recombinant Rabbit mAb
Primary antibody: ARIH1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: PC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64 kDa
Observed MW: 64 kDaWB result of ARIH1 Recombinant Rabbit mAb
Primary antibody: ARIH1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64 kDa
Observed MW: 64 kDaWB result of ARIH1 Recombinant Rabbit mAb
Primary antibody: ARIH1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64 kDa
Observed MW: 64 kDaWB result of ARIH1 Recombinant Rabbit mAb
Primary antibody: ARIH1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64 kDa
Observed MW: 64 kDa
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- ARIH1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- ARIH1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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