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Histone H4 Recombinant Rabbit mAb (S-499-253)

Histone H4,H4/A,H4FA,HIST1H4A,H4C2,H4/I,H4FI,HIST1H4B,H4C3,H4/G,H4FG,HIST1H4C,H4C4,H4/B,H4FB,HIST1H4D,H4C5,H4/J,H4FJ,HIST1H4E,H4C1

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6554
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Histone H4

  • 分子别名

    Histone H4; H4/A; H4FA; HIST1H4A; H4C2; H4/I; H4FI; HIST1H4B; H4C3; H4/G; H4FG; HIST1H4C; H4C4; H4/B; H4FB; HIST1H4D; H4C5; H4/J; H4FJ; HIST1H4E; H4C1

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    P62805

  • 克隆号

    S-499-253

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt, Ck, Rb, Zf, Dg

  • 阳性样本

    HeLa, Jurkat, NIH/3T3, mouse brain, C6, rat brain, zebrafish, dog liver, rabbit liver, pig heart, chicken liver

  • 预测反应种属
    (反应种属缩写表)

    Fu, Dr, Pl, Ys

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms, Rt, Zf, Dg, Rb, Ck, Pg
    IHC-P 1:2000 Hu, Ms, Rt
    ICC 1:500 Hu, Ms
背景介绍

  • Histone H4 is a core component of the nucleosome, the basic structural unit of chromatin in eukaryotic cells, consisting of 102 amino acids that are highly conserved across species and subject to extensive post-translational modifications—such as acetylation, methylation, phosphorylation, and ubiquitination—that collectively regulate chromatin compaction, DNA replication, repair, and transcriptional activity by altering the electrostatic interaction between DNA and the histone octamer or by recruiting specific chromatin-binding proteins.

  • 免疫印迹

    • WB result of Histone H4 Recombinant Rabbit mAb
      Primary antibody: Histone H4 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: Jurkat whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 11 kDa
      Observed MW: 14 kDa

    • WB result of Histone H4 Recombinant Rabbit mAb
      Primary antibody: Histone H4 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Lane 2: mouse brain lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 11 kDa
      Observed MW: 14 kDa

    • WB result of Histone H4 Recombinant Rabbit mAb
      Primary antibody: Histone H4 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Lane 2: rat brain lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 11 kDa
      Observed MW: 14 kDa

    • WB result of Histone H4 Recombinant Rabbit mAb
      Primary antibody: Histone H4 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: zebrafish lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 11 kDa
      Observed MW: 14 kDa

    • WB result of Histone H4 Recombinant Rabbit mAb
      Primary antibody: Histone H4 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: dog liver lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 11 kDa
      Observed MW: 14 kDa

    • WB result of Histone H4 Recombinant Rabbit mAb
      Primary antibody: Histone H4 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: rabbit liver lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 11 kDa
      Observed MW: 14 kDa

    • WB result of Histone H4 Recombinant Rabbit mAb
      Primary antibody: Histone H4 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: chicken liver lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 11 kDa
      Observed MW: 14 kDa

    • WB result of Histone H4 Recombinant Rabbit mAb
      Primary antibody: Histone H4 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: pig heart lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 11 kDa
      Observed MW: 14 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Histone H4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human liver. Anti-Histone H4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-Histone H4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Histone H4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Histone H4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat brian. Anti-Histone H4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-Histone H4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

    • ICC shows positive staining in NIH/3T3 cells. Anti-Histone H4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of Histone H4 in tumor tissue.

    • Expression of Histone H4 in human tissue.

    • Expression of Histone H4 in mouse & rat tissue.

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