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宿主来源
Rabbit抗原名称
SLC8A1分子别名
Sodium/calcium exchanger 1; Na(+)/Ca(2+)-exchange protein 1; Solute carrier family 8 member 1; CNC; NCX1 s免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
P32418克隆号
S-3100-17抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Rt预测反应种属
(反应种属缩写表)GP, Dg, Bv, Ct纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
IF ?
稀释度
应用 稀释度 推荐种属 IHC-P 1:500 Rt IF 1:500 Rt
SLC8A1, also known as NCX1, is a 70-kDa Na⁺/Ca²⁺ exchanger that resides primarily in the sarcolemma of cardiomyocytes, where it catalyzes the electrogenic counter-transport of 3 Na⁺ for 1 Ca²⁺ and thus functions as the dominant Ca²⁺ efflux pathway during diastole; its activity is allosterically regulated by intracellular Ca²⁺, inhibited by intracellular Na⁺ and the lipid PIP₂, and its dysfunction or overexpression contributes to arrhythmias, heart failure, and hypertension, while the gene contains multiple promoters giving rise to tissue-specific splice variants that differ only in their short regulatory N-terminal regions.
免疫组化
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-SLC8A1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-SLC8A1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-SLC8A1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded rat kidney. Anti-SLC8A1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of SLC8A1 in rat tissue.
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